COURSE CONTENTS
UNIT I - INTRODUCTION [9 Sessions]
Definition, importance in Forensic Science; collection and types of evidences for DNA fingerprinting, Genetic basis of DNA Fingerprinting, Chromosomes, DNA, Nuclear DNA and Mitochondrial DNA.
UNIT II-TECHNIQUES OF DNA FINGERPRINTING [9 Sessions]
Isolation, southern blots, radioactive probe, Hybridization reaction, visualization, FTA cards for isolation of DNA.
UNIT III-TYPES OF DNA FINGERPRINTING [9 Sessions]
Single locus DNA fingerprinting, multi–locus DNA Fingerprinting , Mini satellite, micro-satellite, VNTR,, STRs, RFLP.
UNIT IV- POLYMERASE CHAIN REACTION [9 Sessions]
Instrumentation, principle, significance in forensic case samples. Denaturation, annealing and extension, Detection of PCR products.
UNIT V-PRACTICAL APPLICATION OF DNA FINGERPRINTING [9 Sessions]
Paternity and maternity testing, personal identification, criminal identification and Forensic importance; DNA databank, limitations of DNA Fingerprinting, legality of DNA Fingerprinting in India.
- Teacher: Shivani Shrivastav
COURSE CONTENTS
UNIT I - CLASSIFICATION OF EXPLOSIVES AND DETONATORS [9 Sessions]
Primary explosives: lead azide, lead styphnate, mercury fulminate, tetrazene. Secondary explosives: TNT, RDX, PETN, Tetrayl, Gelatines, powders, ANFO, emulsion slurries. Introduction, plain and electric detonators, delay detonators, detonating and safety fuse, high explosive mixtures
UNIT II - DEVELOPMENT OF EXPLOSIVES [9 Sessions]
Development of black powder, nitroglycerine, mercury fulminate, nitrocellulose, dynamite, ammonium nitrate, ANFO. Development of military explosives: picric acid, tetryl, TNT, PETN, RDX and HMX
UNIT III- LOCATION, COLLECTION AND EXAMINATION OF EXPLOSIVE RESIDUE [9 Sessions]
Location and collection of fireworks, home-made bombs, booby traps and letter bombs. Disposal of an explosive device, dispatch of explosive device and exploded material. Chemical examination, Chromatographic techniques: TLC, HPLC, Vapor detection method: adsorption and concentration of explosive vapors.
UNIT IV-LEGAL ASPECTS [9 Sessions]
Explosives Act 1984, (Definition, Powers of Central Govt. and Licensing Authority, Offences and Penalties) and Section 286 of IPC, 1860, (Negligent conduct with respect to explosive substance), Explosive Substances Act 1908, (Definition, Offences and Penalties)
TEXT BOOKS
1. Working Procedure Manual (2000) Chemistry, Explosives and Narcotics, BPR&D Pub
2. D.A. Skoog, D.M. West and F.J. Holler;(1994) Analytical Chemistry: An Introduction; Saunders College Publishing, Philadelphia, USA.
REFERENCE BOOKS
1. Dettean, J D;(2002) Fire Investigation, 5th ed, Prentice Hall, Englewood Cliffs, N.J
2. Y. Lyalikov (1968) Physicochemical Analysis; Mir, Moscow, USS
- Teacher: Shivani Shrivastav